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Subset a SingleCellExperiment to reduce the number of cells

Usage

subsetSCE(
  sce,
  cell_type_col = "celltype_major",
  scenario = "even",
  ncells = 1000,
  notEnough = "asis",
  seed = 12345
)

Arguments

sce

SingleCellExperiment with cell type annotation

cell_type_col

name of colData column containing cell type information

scenario

which fraction of each celltype should be present in the subsampled dataset?

ncells

number of cells in total, defaults to 1000

notEnough

what to do if not enough cells are available c("remove", "asis")

seed

set a seed, enables reproducibility of subsetting step

Value

a singleCellExperiment with